Supplementary Table 1. E.coli genes used for construction of the ArcA binding signal. With the exclusion of one gene this set coincides with that used in (McGuire et al, 1999).

Name

Function

Reference

Experimental method

icdA

isocitrate dehydrogenase, specific for NADP+

Chao et al, 1997

DNase I footprinting and other methods

aldA

aldehyde dehydrogenase, NAD-linked

Pellicer, Lynch et al, 1999

DNA binding assays and other methods

lldP

L-lactate permease

Lynch et al, 1996

DNA binding assays and other methods

cydAB

cytochrome D terminal oxidase, polypeptide subunit I

Lynch et al, 1996

DNA binding assays and other methods

gltA

citrate synthase

Lynch et al, 1996

DNA binding assays and other methods

sdhC

succinate dehydrogenase, hydrophobic subunit

Lynch et al, 1996

DNA binding assays and other methods

sodA

superoxide dismutase, manganese

Hassan et al, 1992

Beta-Galactosidase assays

lpdA

lipoamide dehydrogenase (NADH)

Cunningham et al, 1998

Beta-Galactosidase assays and other methods

glcCD

regulator of degradation of small molecules, carbon compounds

Pellicer, Fernandez et al, 1999

DNA binding assays and other methods

References

Chao,G., Shen,J., Tseng,C.P., Park,S.J., and Gunsalus,R.P. (1997) Aerobic regulation of isocitrate dehydrogenase gene (icd) expression in Escherichia coli by the arcA and fnr gene products. J. Bacteriol., 179 (13), 4299-4304.

Cunningham,L., Georgellis,D., Green,J. and Guest,J.R. (1998) Co-regulation of lipoamide dehydrogenase and 2-oxoglutarate dehydrogenase synthesis in Escherichia coli: characterisation of an ArcA binding site in the lpd promoter. FEMS Microbiol. Lett., 169(2), 403-408.

Hassan,H.M. and Sun,H.C. (1992) Regulatory roles of Fnr, Fur, and Arc in expression of manganese-containing superoxide dismutase in Escherichia coli. Proc. Natl. Acad. Sci. USA, 89 (8), 3217-3221.

Lynch,A.S. and Lin,E.C. (1996) Transcriptional control mediated by the ArcA two-component response regulator protein of Escherichia coli: characterization of DNA binding at target promoters. J. Bacteriol., 178 (21), 6238-6249.

McGuire,A. M., De Wulf,P., Church,G.M. and Lin, E. C. C. (1999) A weight matrix for binding recognition by the redox-response regulator ArcA-P of Escherichia coli. Molecular Microbiology, 32 (1), 219-221.Pellicer,M.T., Fernandez,C., Badia,J., Aguilar,J. Lin,E.C. and Baldom,L. (1999) Cross-induction of glc and ace operons of Escherichia coli attributable to pathway intersection. Characterization of the glc promoter. J. Biol. Chem., 274 (3), 1745-1752.

Pellicer,M.T., Lynch,A.S., De Wulf,P., Boyd,D., Aguilar,J. and Lin,E.C. (1999) A mutational study of the ArcA-P binding sequences in the aldA promoter of Escherichia coli. Mol. Gen. Genet., 261 (1), 170-176.